 =Rhizoglyphus robini

 PoloPlus  Version 2.0
 Date: 30 APR 2026
 Rhizoglyphus robini                                                     
 Data file: C:\Users\Hosein\Desktop\Aba+lead.txt
 Number of preparations: 2
 Number of dose groups: 31
 Model: Probit
 Natural Response Parameter: no
 Convert doses to logarithms: yes
 LDs:  50
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 Rhizoglyphus robini                                                     
  
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 Intercepts and slopes unconstrained. Preparation is ( 1) Aba     
 Not estimating natural response
  
             parameter   standard error   t ratio
 Aba          -1.425          0.169       -8.425
 SLOPE         3.467          0.345       10.044
  
 Variance-Covariance matrix
                 Aba           SLOPE       
   Aba         0.285887E-01 -0.543269E-01
   SLOPE      -0.543269E-01  0.119121    
  
 Chi-squared goodness of fit test
 prep             dose      n       r   expected  residual   probab  std resid
 Aba             5.400     32.     28.     27.76     0.241    0.867     0.126
                 5.400     30.     25.     26.02    -1.024    0.867    -0.551
                 5.400     25.     22.     21.69     0.313    0.867     0.185
                 5.400     28.     24.     24.29    -0.289    0.867    -0.161
                 3.600     26.     18.     18.01    -0.015    0.693    -0.006
                 3.600     34.     24.     23.56     0.442    0.693     0.164
                 3.600     42.     28.     29.10    -1.101    0.693    -0.368
                 3.600     31.     22.     21.48     0.521    0.693     0.203
                 2.400     35.     17.     16.02     0.983    0.458     0.334
                 2.400     35.     16.     16.02    -0.017    0.458    -0.006
                 2.400     30.     15.     13.73     1.271    0.458     0.466
                 2.400     29.     14.     13.27     0.729    0.458     0.272
                 1.600     27.      6.      6.39    -0.391    0.237    -0.177
                 1.600     25.      5.      5.92    -0.918    0.237    -0.432
                 1.600     28.      6.      6.63    -0.628    0.237    -0.279
                 1.600     29.      7.      6.86     0.135    0.237     0.059
  
 chi-square: 1.285     degrees of freedom: 14     heterogeneity: 0.092
  
 Effective Doses
                   dose      limits    0.90     0.95     0.99
 LD50  Aba        2.576      lower    2.393    2.357    2.284
                             upper    2.756    2.792    2.863
  
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 Intercepts and slopes unconstrained. Preparation is ( 2) Aba+Lead
 Not estimating natural response
  
             parameter   standard error   t ratio
 Aba+Lead     -1.602          0.204       -7.843
 SLOPE         3.231          0.376        8.591
  
 Variance-Covariance matrix
                 Aba+Lead      SLOPE       
   Aba+Lead    0.417462E-01 -0.724602E-01
   SLOPE      -0.724602E-01  0.141479    
  
 Chi-squared goodness of fit test
 prep             dose      n       r   expected  residual   probab  std resid
 Aba+Lead        6.400     33.     27.     27.79    -0.785    0.842    -0.375
                 6.400     21.     18.     17.68     0.318    0.842     0.191
                 6.400     22.     18.     18.52    -0.523    0.842    -0.306
                 4.300     20.     15.     13.43     1.567    0.672     0.746
                 4.300     23.     17.     15.45     1.552    0.672     0.689
                 4.300     28.     19.     18.81     0.193    0.672     0.078
                 4.300     26.     16.     17.46    -1.464    0.672    -0.611
                 2.900     28.     12.     12.79    -0.793    0.457    -0.301
                 2.900     19.      7.      8.68    -1.681    0.457    -0.774
                 2.900     30.     15.     13.71     1.293    0.457     0.474
                 2.900     29.     14.     13.25     0.750    0.457     0.280
                 1.900     27.      7.      6.52     0.481    0.241     0.216
                 1.900     25.      6.      6.04    -0.036    0.241    -0.017
                 1.900     28.      8.      6.76     1.239    0.241     0.547
                 1.900     29.      5.      7.00    -2.002    0.241    -0.869
  
 chi-square: 3.775     degrees of freedom: 13     heterogeneity: 0.290
  
 Effective Doses
                   dose      limits    0.90     0.95     0.99
 LD50  Aba+Lead   3.133      lower    2.883    2.834    2.736
                             upper    3.394    3.448    3.560
  
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 HYPOTHESIS OF EQUALITY (equal slopes, equal intercepts): REJECTED (P<0.05)
    (chi-square: 10.16,  degrees of freedom: 2,  tail probability: 0.006)
  
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 HYPOTHESIS OF PARALLELISM (equal slopes): NOT REJECTED (P>0.05)
    (chi-square: 0.21,  degrees of freedom: 1,  tail probability: 0.645)
  
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 Lethal dose ratio (LD50)
              ratio      limits     0.95
 Aba+Lead     0.822      lower     0.723
                         upper     0.936
  
